Mitotic Arrest and Chromosome Doubling Using Thiabendazole, Cambendazole, Nocodazole and Ben Late in the Slime Mould Dictyostelium discoideum
نویسندگان
چکیده
منابع مشابه
A genetic study of aggregation in the cellular slime mould Dictyostelium discoideum using complementation analysis.
A series of aggregation-deficient (aggregateless) mutants were isolated in genetically marked haploid strains of the cellular slime mould Dictyostelium discoideum. Diploids were produced from pairs of such haploid mutants by a fusion system based on this organism's parasexual cycle. The diploids were isolated from the haploids by using complementation of non-allelic growth-temperature-sensitive...
متن کاملAnalysis of optical density wave propagation and cell movement in the cellular slime mould Dictyostelium discoideum
We have studied optical density wave propagation during aggregation of the cellular slime mould Dictyostelium discoideum in a quantitative manner by digital image analysis. The waves arc mostly single ended spiral waves starting from an aggregation center. We can measure a variety of parameters such as oscillation frequency, wave propagation velocity and wave shape. This allows the construction...
متن کاملThe isolation and subfractionation of plasma membrane from the cellular slime mould Dictyostelium discoideum.
A procedure for the isolation and separation of three different subfractions of plasma membrane from the cellular slime mould Dictyostelium discoideum is described. The cells were disrupted by freeze-thawing in liquid N(2) and plasma membranes were purified by equilibrium centrifugation in a sucrose gradient. The cell surface was labelled with radioactive iodide by using the lactoperoxidase iod...
متن کاملExperimentally induced aberrations in the pattern of differentiation in the cellular slime mould Dictyostelium discoideum.
This paper describes a set of perturbatory experiments designed to elucidate aspects of the mechanism by which the normal pattern of differentiation is specified. Experiments are described which investigate the alterations in development seen in aqueous environments, with changes in humidity and with the introduction of permeable and impermeable barriers. The following results are reported: (i)...
متن کاملA bacterial factor induces changes in cysteine proteinase forms in the cellular slime mould Dictyostelium discoideum.
The electrophoretic pattern of cysteine proteinases in axenically grown myxamoebae of Dictyostelium discoideum can be altered by the addition of either Gram-negative (Klebsiella aerogenes, Escherichia coli) or Gram-positive (Micrococcus lysodeikticus, Bacillus subtilis) bacteria to the culture. No changes occurred, however, if either yeast or latex beads were used in place of bacteria. The chan...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1980
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-116-2-397